7K97
Human DNA polymerase beta dGDP product complex with Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-05 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.102, 77.309, 55.111 |
Unit cell angles | 90.00, 114.85, 90.00 |
Refinement procedure
Resolution | 30.580 - 2.400 |
R-factor | 0.1907 |
Rwork | 0.185 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.009 |
RMSD bond angle | 1.089 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.19rc5_4047) |
Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.093 | 0.961 |
Rpim | 0.043 | 0.517 |
Number of reflections | 16511 | 804 |
<I/σ(I)> | 15.3 | 1.2 |
Completeness [%] | 99.5 | 97.3 |
Redundancy | 4.2 | 2.8 |
CC(1/2) | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 50 mM imidazole, 350 mM sodium acetate, 18% PEG3350 |