7K95
Crystal structure of human CPSF30 in complex with hFip1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 64 |
| Unit cell lengths | 79.040, 79.040, 48.662 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.550 - 1.900 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.20920 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.984 |
| Data reduction software | XDS (20180808) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.660 | 48.660 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.046 | 0.034 | 0.874 |
| Rmeas | 0.048 | 0.035 | 0.917 |
| Rpim | 0.013 | ||
| Total number of observations | 174010 | ||
| Number of reflections | 13793 | 137 | 869 |
| <I/σ(I)> | 30.9 | ||
| Completeness [%] | 100.0 | 98.6 | 99.9 |
| Redundancy | 12.6 | 11.6 | 10.9 |
| CC(1/2) | 1.000 | 0.999 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 0.1 M sodium malonate, 16% (w/v) PEG 3350 |
