7K86
Crystal Structure of Glutamyl-tRNA synthetase (gltX) from Stenotrophomonas maltophilia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-05 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.400, 69.110, 103.820 |
Unit cell angles | 90.00, 110.06, 90.00 |
Refinement procedure
Resolution | 36.260 - 2.050 |
R-factor | 0.1833 |
Rwork | 0.182 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h4v |
RMSD bond length | 0.006 |
RMSD bond angle | 0.802 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.260 | 36.260 | 2.100 |
High resolution limit [Å] | 2.050 | 9.170 | 2.050 |
Rmerge | 0.039 | 0.025 | 0.434 |
Rmeas | 0.045 | 0.030 | 0.523 |
Total number of observations | 293786 | ||
Number of reflections | 71304 | 826 | 5087 |
<I/σ(I)> | 18.49 | 43.47 | 2.42 |
Completeness [%] | 99.6 | 96.3 | 97.4 |
Redundancy | 4.12 | 3.735 | 3.23 |
CC(1/2) | 0.999 | 0.998 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 287 | StmaA.01187.b.B1.PW38726 at 24 mg/ml mixed 1:1 with MCSG1(c5): 10%(w/v) PEG-3350, 0.2 M magensium acetate. Tray: 313794c5. Puck: xku5-10. |