7K7P

Structure of SARS-CoV-2 nonstuctural protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	103
Detector technology	PIXEL
Collection date	2020-09-08
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	36.589, 36.589, 141.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.420 - 1.770
R-factor	0.1914
Rwork	0.188
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hsx
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.420	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.071	0.634
Rmeas	0.073	0.743
Rpim	0.018	0.376
Number of reflections	10106	965
<I/σ(I)>	40.38
Completeness [%]	99.8
Redundancy	13.5
CC(1/2)	0.998	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30 % w/v Polyethylene glycol 8,000