7K7M
Crystal Structure of a membrane protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.378, 147.710, 138.192 |
| Unit cell angles | 90.00, 120.89, 90.00 |
Refinement procedure
| Resolution | 98.660 - 3.330 |
| R-factor | 0.2696 |
| Rwork | 0.267 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6or2 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.460 |
| High resolution limit [Å] | 3.330 | 7.200 | 3.340 |
| Rmerge | 0.089 | 0.031 | 3.041 |
| Rmeas | 0.101 | 0.035 | 3.442 |
| Rpim | 0.046 | 0.016 | 1.578 |
| Number of reflections | 44875 | 4554 | 4427 |
| <I/σ(I)> | 5.2 | ||
| Completeness [%] | 99.1 | 98.3 | 99.3 |
| Redundancy | 4.6 | 4.5 | 4.6 |
| CC(1/2) | 0.998 | 0.170 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 20mM Tris, 100mM NaAC and 25% PEG400 |
