7K7J

EphB6 receptor ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97920
Spacegroup name	P 43 21 2
Unit cell lengths	105.310, 105.310, 188.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.055 - 3.004
R-factor	0.2371
Rwork	0.235
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fl7
RMSD bond length	0.011
RMSD bond angle	1.423
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	21919	1066
<I/σ(I)>	20.1
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.999	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M sodium nitrate, 0.1 M sodium acetate trihydrate pH 4.6