7K5U

Bst DNA polymerase I time-resolved structure, 48 hr post dATP and dCTP addition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2020-06-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.299, 93.491, 105.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.060 - 2.000
R-factor	0.1907
Rwork	0.190
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsy
RMSD bond length	0.007
RMSD bond angle	0.842
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.060	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.064	0.164
Rmeas	0.070	0.180
Rpim	0.028	0.074
Number of reflections	58064	5685
<I/σ(I)>	20.31
Completeness [%]	98.2
Redundancy	6.3
CC(1/2)	0.997	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD