7K5T

Bst DNA polymerase I time-resolved structure, 25.5 hr post dATP and dCTP addition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	86.631, 93.589, 105.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.790 - 2.300
R-factor	0.1932
Rwork	0.191
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsy
RMSD bond length	0.008
RMSD bond angle	0.881
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.790	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.062	0.480
Rmeas	0.068	0.520
Rpim	0.027	0.198
Number of reflections	38768	3819
<I/σ(I)>	19.23
Completeness [%]	99.8
Redundancy	6.5
CC(1/2)	0.999	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD