7K5S
Bst DNA polymerase I time-resolved structure, 4 hr post dATP and dCTP addition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.622, 93.416, 105.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.050 - 1.670 |
R-factor | 0.1872 |
Rwork | 0.187 |
R-free | 0.20960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dsy |
RMSD bond length | 0.006 |
RMSD bond angle | 0.815 |
Data reduction software | autoXDS |
Data scaling software | autoXDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.050 | 1.730 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.055 | 0.807 |
Rmeas | 0.060 | 0.873 |
Rpim | 0.024 | 0.330 |
Number of reflections | 99196 | 9747 |
<I/σ(I)> | 18.14 | |
Completeness [%] | 98.1 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50% ammonium sulfate, 0.1M MES, 2% MPD |