7K5R

Bst DNA polymerase I time-resolved structure, 120 min post dATP addition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.801, 93.188, 104.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.720 - 2.300
R-factor	0.1907
Rwork	0.188
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsy
RMSD bond length	0.008
RMSD bond angle	0.901
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.720	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.141	0.993
Rmeas	0.154	1.097
Rpim	0.059	0.460
Number of reflections	38829	3812
<I/σ(I)>	13.73
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	0.997	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD