7K5P
Bst DNA polymerase I time-resolved structure, 4 min post dATP addition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.540, 93.301, 105.494 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.350 - 1.970 |
R-factor | 0.2201 |
Rwork | 0.219 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dsy |
RMSD bond length | 0.008 |
RMSD bond angle | 0.924 |
Data reduction software | autoXDS |
Data scaling software | autoXDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.350 | 2.040 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.048 | 0.186 |
Rmeas | 0.055 | 0.210 |
Rpim | 0.026 | 0.096 |
Number of reflections | 60952 | 6101 |
<I/σ(I)> | 21.97 | |
Completeness [%] | 97.7 | |
Redundancy | 4.6 | |
CC(1/2) | 0.998 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50% ammonium sulfate, 0.1M MES, 2% MPD |