7K5O

Bst DNA polymerase I time-resolved structure, 1 min post dATP addition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	86.990, 93.050, 104.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.890 - 2.160
R-factor	0.1881
Rwork	0.186
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsy
RMSD bond length	0.008
RMSD bond angle	0.895
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.890	2.237
High resolution limit [Å]	2.160	2.160
Number of reflections	46274	4541
<I/σ(I)>	12.5
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.997	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% saturated ammonium sulfate, 0.1M MES, 2% MPD