7K5O
Bst DNA polymerase I time-resolved structure, 1 min post dATP addition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.990, 93.050, 104.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.890 - 2.160 |
R-factor | 0.1881 |
Rwork | 0.186 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dsy |
RMSD bond length | 0.008 |
RMSD bond angle | 0.895 |
Data reduction software | autoXDS |
Data scaling software | autoXDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.890 | 2.237 |
High resolution limit [Å] | 2.160 | 2.160 |
Number of reflections | 46274 | 4541 |
<I/σ(I)> | 12.5 | |
Completeness [%] | 99.9 | |
Redundancy | 7 | |
CC(1/2) | 0.997 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50% saturated ammonium sulfate, 0.1M MES, 2% MPD |