7K5L

Ebola virus VP40 octameric ring generated by an RNA oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97931
Spacegroup name	P 4 2 2
Unit cell lengths	80.626, 80.626, 47.083
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.540 - 1.380
R-factor	0.181
Rwork	0.180
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ldm
RMSD bond length	0.005
RMSD bond angle	0.784
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.630	1.410
High resolution limit [Å]	1.380	1.380
Rmerge	0.059	1.519
Number of reflections	30977	1580
<I/σ(I)>	27.8	2.4
Completeness [%]	95.7	100
Redundancy	24.8	25.7
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	1 uL 3.5 mg/mL protein, 1 uL 75 mM sodium citrate tribasic pH 8.4, 150 mM ammonium acetate