7K59
Structure of apo VCP hexamer generated from bacterially recombinant VCP/p97
EM specimen
Vitrification | Yes |
Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum LS |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.2 |
Number of Particle | 71600 |
Software | Gautomatch,cryoSPARC,Leginon,Gctf |
Symmetry | C6 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |