7K56
Structure of VCP dodecamer purified from H1299 cells
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.9 |
| Number of Particle | 40000 |
| Software | Gautomatch,cryoSPARC,Leginon,Gctf |
| Symmetry | D6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
