7K3Z
P. falciparum Cpn60 D474A mutant bound to ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-25 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 281.498, 281.498, 298.827 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.990 - 3.690 |
| R-factor | 0.2279 |
| Rwork | 0.227 |
| R-free | 0.27650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v4o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.803 |
| Data reduction software | XDS (Version Jan. 31, 2020) |
| Data scaling software | XDS (Version Jan. 31, 2020) |
| Phasing software | PHENIX (1.14_3260) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.000 | 3.790 |
| High resolution limit [Å] | 3.690 | 3.690 |
| Rmeas | 0.133 | 1.500 |
| Number of reflections | 75184 | 5368 |
| <I/σ(I)> | 12.93 | 1.7 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 9.1 | |
| CC(1/2) | 0.999 | 0.607 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 291 | 100 mM phosphate citrate buffer pH 4.4, 13% Isopropanol, and 0.21 mM of Lithium Sulfate |
