7K3U
X-ray crystallographic structure model of Lactococcus lactis prolidase mutant R293S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 146.300, 80.000, 187.150 |
Unit cell angles | 90.00, 102.03, 90.00 |
Refinement procedure
Resolution | 49.190 - 2.700 |
R-factor | 0.2029 |
Rwork | 0.198 |
R-free | 0.28820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zng |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.190 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 116597 | 11587 |
<I/σ(I)> | 10.8 | |
Completeness [%] | 99.7 | |
Redundancy | 3.4 | |
CC(1/2) | 0.998 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 0.1 M calcium acetate, 0.1 M Bis-Tris propane, 15% w/v PEG4000 |