7K3N

Crystal Structure of NSP1 from SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97913
Spacegroup name	P 43 21 2
Unit cell lengths	37.007, 37.007, 144.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.950 - 1.650
R-factor	0.2076
Rwork	0.203
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hsx
RMSD bond length	0.006
RMSD bond angle	0.872
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PARROT
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	13023	642
<I/σ(I)>	35.6
Completeness [%]	99.8
Redundancy	8.2
CC(1/2)	0.997	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292.15	0.2 M sodium formate, 20% PEG3350