7K3L
Crystal structure of dLC8 in complex with Panoramix TQ peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 62.900, 62.900, 120.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.750 - 1.790 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.20950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zke |
RMSD bond length | 0.007 |
RMSD bond angle | 0.857 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.200 | 1.890 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.093 | 0.415 |
Number of reflections | 11305 | 1281 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 96.1 | |
Redundancy | 8.6 | |
CC(1/2) | 0.997 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M KSCN, 30% (w/v) PEG 2000 MME |