7K30

Crystal structure of Endonuclease Q complex with 27-mer duplex substrate with dU at the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	152.700, 152.700, 118.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	88.120 - 2.340
R-factor	0.2067
Rwork	0.205
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zb8
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.120	88.120	2.400
High resolution limit [Å]	2.320	8.990	2.320
Rmerge	0.102	0.046	9.900
Rmeas	0.106	0.048
Rpim	0.031	0.014	3.346
Total number of observations	509530
Number of reflections	44238	765	4311
<I/σ(I)>	11.2
Completeness [%]	99.5	99.2	98.9
Redundancy	11.5	11.1	11
CC(1/2)	0.999	0.999	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	295	0.2 M tri-potassium citrate and 15 % (w/v) polyethylene glycol (PEG) 3,350