7K26

Crystal structure of Human H-chain Ferritin variant infused with Sodium Acrylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9801
Spacegroup name	P 31 2 1
Unit cell lengths	131.570, 131.570, 306.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.990 - 2.700
R-factor	0.2009
Rwork	0.198
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b8f
RMSD bond length	0.005
RMSD bond angle	0.733
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.440	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	85108	4498
<I/σ(I)>	8.5
Completeness [%]	99.8
Redundancy	18
CC(1/2)	0.996	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Reservoir: 525 uL total volume 100 uL of 500 mM HEPES pH 7.0, 125 uL of 1 M NH4OAc, 300 uL 2-Methyl-2,4-pentanediol Sitting drop: 5 uL reservoir, 5 uL of 25 uM dCs ferritin