7K1P
Crystal structure of the second bromodomain (BD2) of human TAF1 bound to bromosporine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-17 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 95.186, 95.186, 94.957 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.220 - 2.450 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jjg |
Data reduction software | DIALS |
Data scaling software | Aimless (0.5.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.220 | 41.220 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.054 | 0.501 |
Number of reflections | 18720 | 18720 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 11 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES (pH 7.5), 10% PEG 8,000, 8% Ethylene glycol |