7K1P

Crystal structure of the second bromodomain (BD2) of human TAF1 bound to bromosporine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-17
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	95.186, 95.186, 94.957
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.220 - 2.450
R-factor	0.195
Rwork	0.194
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jjg
Data reduction software	DIALS
Data scaling software	Aimless (0.5.1)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.220	41.220
High resolution limit [Å]	2.450	2.450
Rmerge	0.054	0.501
Number of reflections	18720	18720
<I/σ(I)>	13.4
Completeness [%]	100.0	100
Redundancy	11	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES (pH 7.5), 10% PEG 8,000, 8% Ethylene glycol