7K16
Tamana Bat Virus xrRNA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-02-25 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0972 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 79.100, 79.100, 40.690 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.550 - 2.100 |
R-factor | 0.1918 |
Rwork | 0.187 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oiu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.239 |
Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20191015) |
Data scaling software | XDS (VERSION Mar 15, 2019 BUILT=20191015) |
Phasing software | PHENIX (1.16_3549) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.550 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.095 | 1.694 |
Rmeas | 0.097 | 1.739 |
Rpim | 0.021 | 0.391 |
Number of reflections | 8806 | 875 |
<I/σ(I)> | 27.9 | 2.15 |
Completeness [%] | 99.9 | 100 |
Redundancy | 21.2 | 19.6 |
CC(1/2) | 1.000 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Mg2+chloride hexahydrate, 0.1M HEPES sodium (pH 7.5), and 30% v/v Polyethylene glycol 400 |