7K0Z

Marsupial T cell receptor Spl_157

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-10
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	72.328, 110.722, 207.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 3.200
R-factor	0.2585
Rwork	0.257
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished
RMSD bond length	0.003
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.030	3.390
High resolution limit [Å]	3.200	3.200
Rpim	0.060	0.545
Number of reflections	28367	4496
<I/σ(I)>	10.6	1.6
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M CBTP pH 8.8, 15-20% PEG 3350