7K07
Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-22 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.005 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.627, 74.777, 96.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.390 - 2.150 |
R-factor | 0.1791 |
Rwork | 0.177 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xri |
RMSD bond length | 0.005 |
RMSD bond angle | 0.742 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.400 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmeas | 0.161 | |
Rpim | 0.051 | |
Number of reflections | 44601 | 2173 |
<I/σ(I)> | 39.2 | |
Completeness [%] | 99.8 | |
Redundancy | 9 | |
CC(1/2) | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 35% MPEG 2K, 20% saturated KCl, 200 mM pH 8.0 Imidazole Malate, 5mM MnCl2, 20mM AMP |