7JZI

Crystal structure of LAIR1 ectodomain (from MGD21) in complex with Plasmodium RIFIN (PF3D7_1040300) V2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0722
Spacegroup name	C 1 2 1
Unit cell lengths	144.409, 72.178, 57.917
Unit cell angles	90.00, 91.08, 90.00

Refinement procedure

Resolution	44.761 - 2.707
R-factor	0.2438
Rwork	0.240
R-free	0.28070
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.318
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.070	0.053	0.593
Rmeas	0.075	0.058	0.647
Rpim	0.027	0.022	0.253
Number of reflections	31123	745	768
<I/σ(I)>	13.1
Completeness [%]	98.8	87.3	94.6
Redundancy	7.2	6.6	5.6
CC(1/2)		0.997	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.085 M Na HEPES pH 7.5, 17% PEG 4000, 15% Glycerol, 8.5% Isopropanol