7JZI
Crystal structure of LAIR1 ectodomain (from MGD21) in complex with Plasmodium RIFIN (PF3D7_1040300) V2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0722 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.409, 72.178, 57.917 |
Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
Resolution | 44.761 - 2.707 |
R-factor | 0.2438 |
Rwork | 0.240 |
R-free | 0.28070 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.318 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.070 | 0.053 | 0.593 |
Rmeas | 0.075 | 0.058 | 0.647 |
Rpim | 0.027 | 0.022 | 0.253 |
Number of reflections | 31123 | 745 | 768 |
<I/σ(I)> | 13.1 | ||
Completeness [%] | 98.8 | 87.3 | 94.6 |
Redundancy | 7.2 | 6.6 | 5.6 |
CC(1/2) | 0.997 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.085 M Na HEPES pH 7.5, 17% PEG 4000, 15% Glycerol, 8.5% Isopropanol |