7JZG
Dihydrodipicolinate synthase mutant S48F with lysine in the allosteric site and pyruvate in the catalytic site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 294.15 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.189, 121.189, 110.162 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.340 - 1.820 |
| R-factor | 0.13841 |
| Rwork | 0.137 |
| R-free | 0.16888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.283 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.340 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.086 | 0.329 |
| Number of reflections | 83589 | 4524 |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294.15 | glucopyranoside, potassium phosphate dibasic |
