7JZD
Dihydrodipicolinate synthase mutation S48W with Lysine in the allosteric site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 294.15 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.073, 121.073, 111.804 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.660 - 1.910 |
| R-factor | 0.1604 |
| Rwork | 0.159 |
| R-free | 0.18925 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.118 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.070 | 1.950 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.076 | 0.614 |
| Rpim | 0.035 | |
| Number of reflections | 73768 | 4271 |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294.15 | glucopyranoside, potassium phosphate dibasic |
