7JZ8
Dihydrodipicolinate synthase mutant S48F with lysine in the allosteric site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 294.15 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 121.049, 121.049, 110.151 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.310 - 1.820 |
R-factor | 0.14468 |
Rwork | 0.143 |
R-free | 0.16984 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yxc |
RMSD bond length | 0.024 |
RMSD bond angle | 2.168 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.300 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.137 | |
Number of reflections | 83378 | 4541 |
<I/σ(I)> | 11.7 | |
Completeness [%] | 100.0 | |
Redundancy | 10.8 | |
CC(1/2) | 0.846 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 249.15 | glucopyrdoside, Dipotassium phosphate |