7JZ7

Dihydrodipicolinate synthase mutant S48F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	294.15
Detector technology	CCD
Collection date	2011-11-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95369
Spacegroup name	P 31 2 1
Unit cell lengths	120.729, 120.729, 110.812
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.170 - 1.740
R-factor	0.15022
Rwork	0.149
R-free	0.17205
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yxc
RMSD bond length	0.026
RMSD bond angle	2.250
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	1.770
High resolution limit [Å]	1.740	1.740
Rmerge	0.133
Rpim	0.042
Number of reflections	95335	4536
<I/σ(I)>	10.5
Completeness [%]	100.0
Redundancy	10.6
CC(1/2)		0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294.15	glucopyranoside, Dipotassium phosphate