7JZ5
Cellular retinol-binding protein 2 (CRBP2) in complex with 1-arachodonoyl-1-thio-glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-07-14 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.816, 67.562, 89.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.918 - 1.567 |
R-factor | 0.161702646311 |
Rwork | 0.161 |
R-free | 0.18309 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bti |
RMSD bond length | 0.008 |
RMSD bond angle | 1.034 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.480 | 1.590 |
High resolution limit [Å] | 1.567 | 1.570 |
Rmerge | 0.089 | 0.689 |
Rmeas | 0.111 | 0.818 |
Rpim | 0.055 | 0.432 |
Number of reflections | 49723 | 2289 |
<I/σ(I)> | 14.1 | 2.1 |
Completeness [%] | 99.6 | 93.8 |
Redundancy | 6.5 | |
CC(1/2) | 0.994 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris HCl, PEG 3350 22-28% |