7JZ1

Crystal structure of broadly Plasmodium RIFIN reactive LAIR1-inserted antibody MGC34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	101.379, 101.379, 319.878
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.330 - 3.370
R-factor	0.2391
Rwork	0.238
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kgr
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.470
High resolution limit [Å]	3.350	7.210	3.350
Rmerge	0.177	0.097	0.784
Rmeas	0.202	0.112	0.909
Rpim	0.094	0.055	0.445
Total number of observations	62324
Number of reflections	14664	1646	1416
<I/σ(I)>	3.7
Completeness [%]	99.8	99.1	100
Redundancy	4.3	3.9	3.7
CC(1/2)		0.988	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MgCl2 hexahydrate, 0.1 M Tris HCl pH 8.5, 20% PEG 3350, 25% PEG 400