7JYO
JAK2 JH2 in complex with JAK064
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-12 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.568, 57.375, 61.071 |
| Unit cell angles | 90.00, 111.00, 90.00 |
Refinement procedure
| Resolution | 40.442 - 2.161 |
| R-factor | 0.188225496472 |
| Rwork | 0.185 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5usz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.805 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmeas | 0.068 | 0.233 |
| Rpim | 0.038 | 0.143 |
| Number of reflections | 15316 | 1383 |
| <I/σ(I)> | 15.2 | 2.95 |
| Completeness [%] | 98.5 | 89.5 |
| Redundancy | 3 | 2.1 |
| CC(1/2) | 0.979 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 0.1M TRIS, PH 8.0 0.2M SODIUM ACETATE, 0.001 M TCEP, 12-24% PEG 4,000 |
