7JXV

ANTH domain of CALM (clathrin-assembly lymphoid myeloid leukemia protein) bound to ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-11-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 41 21 2
Unit cell lengths	94.068, 94.068, 91.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.830 - 2.350
R-factor	0.2093
Rwork	0.206
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 3zyk
RMSD bond length	0.014
RMSD bond angle	1.633
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.830	18.830	2.440
High resolution limit [Å]	2.350	8.790	2.350
Rmerge	0.096	0.039	0.940
Rmeas	0.100	0.042	0.975
Rpim	0.027	0.013	0.260
Total number of observations	238917	3049	24333
Number of reflections	17581	360	1749
<I/σ(I)>	22.1	50.2	3.2
Completeness [%]	99.4	90	96.5
Redundancy	13.6	8.5	13.9
CC(1/2)	0.999	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1 M MES, pH 6, 0.2 M NaCl, 20% w/v MPEG2000