7JXN
Beta hairpin derived from Abeta17-36 with an F20Cha mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 123.15 |
| Detector technology | CCD |
| Collection date | 2019-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 |
| Unit cell lengths | 37.167, 37.167, 116.994 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.190 - 2.000 |
| R-factor | 0.2827 |
| Rwork | 0.277 |
| R-free | 0.30120 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_3908) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.190 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.013 | |
| Rmeas | 0.018 | |
| Rpim | 0.013 | |
| Number of reflections | 12449 | 1258 |
| <I/σ(I)> | 33.1 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.4 | 19.5 |
| CC(1/2) | 1.000 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297.15 | Bis-Tris buffer, ammonium acetate, and methyl-2,4-pentanediol |
