7JX7

BRD2-BD2 in complex with a diacetylated-H2A.Z peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 2 21 21
Unit cell lengths	32.190, 52.457, 71.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.350 - 1.750
R-factor	0.1856
Rwork	0.184
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oni
RMSD bond length	0.006
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.350	1.750
High resolution limit [Å]	1.750	1.750
Rmerge	0.290	0.290
Number of reflections	12845	680
<I/σ(I)>	9.3
Completeness [%]	100.0
Redundancy	14.4
CC(1/2)	0.987	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	24% w/v PEG 1500, 20% w/v glycerol