7JX6

STRUCTURE OF THE SARS-CoV-2 ORF8 ENCODED ACCESSORY PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-07-23
Detector	RDI CMOS_8M
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	50.647, 50.647, 168.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.020 - 1.610
Rwork	0.202
R-free	0.23310
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.020	1.668
High resolution limit [Å]	1.610	1.610
Rmerge	0.057	1.631
Rmeas	0.059	1.696
Number of reflections	29522	2877
<I/σ(I)>	27.09	1.27
Completeness [%]	99.8	99.86
Redundancy	13.4	13.4
CC(1/2)	1.000	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2M Ammonium Chloride, 20% PEG 3350