7JX2
Cellular retinol-binding protein 2 (CRBP2) in complex with 2-palmitoylglycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-03-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.339, 64.450, 66.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.310 - 1.800 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bti |
RMSD bond length | 0.008 |
RMSD bond angle | 0.789 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.580 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.113 | 0.873 |
Rmeas | 0.134 | |
Rpim | 0.072 | 0.554 |
Number of reflections | 15466 | 889 |
<I/σ(I)> | 8.2 | 2.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.3 | 6.6 |
CC(1/2) | 0.996 | 0.881 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris HCl, PEG 3350 22-28% |