7JWD

Cellular retinol-binding protein 2 (CRBP2) in complex with 2-linoleoylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-11-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	57.720, 67.659, 88.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.391 - 1.350
R-factor	0.118177218425
Rwork	0.117
R-free	0.14835
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bti
RMSD bond length	0.009
RMSD bond angle	0.962
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.720	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.061	0.299
Rmeas	0.073	0.369
Rpim	0.040	0.211
Number of reflections	71215	2776
<I/σ(I)>	14.1	3.7
Completeness [%]	93.0	72.7
Redundancy	5.7	4.9
CC(1/2)	0.997	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris HCl, PEG 3350 22-28%