7JW4

Crystal structure of PdGH110B in complex with D-galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-02
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.514
Spacegroup name	C 1 2 1
Unit cell lengths	169.786, 128.093, 100.014
Unit cell angles	90.00, 123.16, 90.00

Refinement procedure

Resolution	24.982 - 2.342
R-factor	0.187
Rwork	0.185
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Wildtype PdGH110B
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.342	2.350
Number of reflections	74084	4528
<I/σ(I)>	10.4
Completeness [%]	99.6
Redundancy	4
CC(1/2)	0.989	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	PEG 6000, HEPES, MPD