7JW3
Crystal structure of Aedes aegypti Nibbler NTD domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 62 |
| Unit cell lengths | 219.224, 219.224, 39.382 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.210 - 3.050 |
| R-factor | 0.2373 |
| Rwork | 0.236 |
| R-free | 0.26690 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.159 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.160 | 3.551 |
| Rmeas | 0.164 | 3.642 |
| Rpim | 0.036 | 0.805 |
| Number of reflections | 21285 | 2058 |
| <I/σ(I)> | 18.12 | 1.05 |
| Completeness [%] | 99.7 | 99.28 |
| Redundancy | 20.2 | 20.1 |
| CC(1/2) | 0.999 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M sodium tartrate, 0.1 M HEPES, pH 7.0, 20% PEG3350 |
