7JVS
Crystal Structure of an Essential Ribosomal Processing Protease Prp from S. aureus in complex with a Substrate Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-17 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54056 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.552, 56.824, 130.278 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.840 - 2.300 |
R-factor | 0.2266 |
Rwork | 0.224 |
R-free | 0.26410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4peo |
RMSD bond length | 0.017 |
RMSD bond angle | 1.536 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.840 | 28.840 | 2.390 |
High resolution limit [Å] | 2.300 | 8.610 | 2.300 |
Rmerge | 0.180 | 0.084 | 1.370 |
Rmeas | 0.185 | 0.087 | 1.416 |
Rpim | 0.043 | 0.022 | 0.353 |
Number of reflections | 9024 | 205 | 941 |
<I/σ(I)> | 10.8 | 24.8 | 2.1 |
Completeness [%] | 100.0 | 98 | 100 |
Redundancy | 18.6 | 14.5 | 15.8 |
CC(1/2) | 0.998 | 0.998 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 1000, Hepes, CaCl2 |