7JVO

Importin alpha bound to the C-terminus of ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-04-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.938, 89.948, 100.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 2.200
R-factor	0.1874
Rwork	0.186
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvt
RMSD bond length	0.004
RMSD bond angle	0.665
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.980	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.095
Number of reflections	36873	3125
<I/σ(I)>	15.9
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.999	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	600 mM citrate, pH 7.0