7JVD

Fab of 5.6 monoclonal mouse IgG1 co-crystallized with the trisaccharide form of serotype 3 pneumococcal capsular polysaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	49.516, 134.302, 133.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.100 - 2.300
R-factor	0.1872
Rwork	0.183
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xjm
RMSD bond length	0.009
RMSD bond angle	1.155
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.270	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	20227	1971
<I/σ(I)>	14.6	2.5
Completeness [%]	100.0	100
Redundancy	13	13.7
CC(1/2)	0.999	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	0.1 M Sodium chloride, 0.1 M BICINE pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 550