7JUM

Pfs230 D1 domain in complex with neutralizing antibody LMIV230-01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	144.020, 144.020, 54.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.739 - 1.998
Rwork	0.191
R-free	0.23310
Structure solution method	AB INITIO PHASING
RMSD bond length	0.008
RMSD bond angle	1.463
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.740	2.070
High resolution limit [Å]	1.998	2.000
Number of reflections	85033	5629
<I/σ(I)>	5.34
Completeness [%]	99.4
Redundancy	3.22
CC(1/2)	0.980	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES, pH 6.5, 26 % PEG 3350