7JUH

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with Man-alpha1-6Man

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	31.922, 59.040, 34.846
Unit cell angles	90.00, 114.48, 90.00

Refinement procedure

Resolution	31.700 - 1.400
R-factor	0.1541
Rwork	0.152
R-free	0.18570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	partially refined model in different symmetry
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.700	31.710	1.420
High resolution limit [Å]	1.400	7.660	1.400
Rmerge	0.046	0.038	0.242
Rmeas	0.050	0.041	0.263
Rpim	0.019	0.016	0.102
Number of reflections	22757	155	1069
<I/σ(I)>	16.9
Completeness [%]	98.0	99.6	96.5
Redundancy	6.8	7	6.4
CC(1/2)	0.999	0.993	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6.0mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 30mM Man-alpha1-6-Man; Reservoir solution: 15% Peg 400, 0.1M Mes pH=6.0