7JUF

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with Man-alpha1-2Man

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	34.414, 56.376, 61.370
Unit cell angles	90.00, 100.92, 90.00

Refinement procedure

Resolution	33.800 - 1.400
R-factor	0.136
Rwork	0.134
R-free	0.17050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	partially refined model in different symmetry
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.800	33.790	1.420
High resolution limit [Å]	1.400	7.660	1.400
Rmerge	0.042	0.031	0.123
Rmeas	0.049	0.036	0.145
Rpim	0.025	0.019	0.075
Total number of observations		1132	7590
Number of reflections	44557	303	2142
<I/σ(I)>	18	29.6	7.9
Completeness [%]	98.0	99.3	95.8
Redundancy	3.6	3.7	3.5
CC(1/2)	0.998	0.998	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6.0mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and30 mM Man-alpha1-2-Man; Reservoir solution: 15% Peg 400, 0.1M Mes pH=6.0