7JUE

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with Man-alpha1-2Man

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	34.227, 54.254, 35.714
Unit cell angles	90.00, 95.37, 90.00

Refinement procedure

Resolution	34.080 - 1.400
R-factor	0.1407
Rwork	0.139
R-free	0.16650
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	partially refined model from a different complex
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.560	35.560	1.420
High resolution limit [Å]	1.400	7.660	1.400
Rmerge	0.040	0.030	0.143
Rmeas	0.043	0.032	0.155
Rpim	0.016	0.012	0.059
Total number of observations		1228	8109
Number of reflections	24845	174	1205
<I/σ(I)>	25.4	46.2	8.3
Completeness [%]	96.6	99.4	93.6
Redundancy	7	7.1	6.7
CC(1/2)	0.999	0.997	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 5.0mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 30mM Man-alpha1-2-Man; Reservoir solution: 15% Peg 400, 0.1M Mes pH=6.0