7JUC
C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with methyl-mannoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.268, 54.005, 35.525 |
Unit cell angles | 90.00, 95.86, 90.00 |
Refinement procedure
Resolution | 29.570 - 1.400 |
R-factor | 0.1366 |
Rwork | 0.135 |
R-free | 0.16010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | partially refined model in different symmetry |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.340 | 35.340 | 1.420 |
High resolution limit [Å] | 1.400 | 7.660 | 1.400 |
Rmerge | 0.047 | 0.061 | 0.082 |
Rmeas | 0.051 | 0.066 | 0.089 |
Rpim | 0.019 | 0.024 | 0.034 |
Total number of observations | 1210 | 8059 | |
Number of reflections | 25015 | 173 | 1233 |
<I/σ(I)> | 22 | 36.5 | 10.3 |
Completeness [%] | 98.0 | 99.4 | 94.6 |
Redundancy | 6.9 | 7 | 6.5 |
CC(1/2) | 0.999 | 0.996 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Protein solution: 6.0mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 50mM Me-Man Reservoir solution: 10% Peg 400, 0.1M Mes pH=6.0 |