7JUB

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with methyl-mannoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	32.970, 56.361, 34.217
Unit cell angles	90.00, 112.01, 90.00

Refinement procedure

Resolution	31.700 - 1.200
R-factor	0.1353
Rwork	0.134
R-free	0.15710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	partially refined model in different symmetry
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.700	31.700	1.220
High resolution limit [Å]	1.200	6.570	1.200
Rmerge	0.075	0.126	0.078
Rmeas	0.081	0.137	0.085
Rpim	0.031	0.051	0.034
Number of reflections	35621	238	1729
<I/σ(I)>	20.9
Completeness [%]	98.1	99.8	95.9
Redundancy	6.7	7	6.2
CC(1/2)	0.995	0.978	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 7.5mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 50mM Me-Man. Reservoir solution: 15% Peg 8K, 0.1M Mes pH=6.0